TALEs, Zinc Fingers, or Meganucleases - still competitive with CRISPrs

May 2013. Conventional breeding still uses nuclear energy which is a standard to induce mutations in plants. Best known is triticum durum used to produce pasta products. While gene-infiltration techniques with agrobacterium tumefaciens are coming of age after thirty years, novel molecular biological methods emerge. These allow plant engineering with yet unknown surgical precision avoiding imprecisions and undesired mutations caused by radioactive irradiation. New genome editing are said to speed up crop development to market, but how? How do companies cope with licensing restrictions of applications? Will there be an impact on the trench warfare between defenders and detractors of GM-crops? Johan Botterman, Head of Product Research Bayer CropScience NV shares his professional experience and opinion with readers of B2Bioworld.

Update TALE’s are still competitive with CRISPr. You need to know when to use one or the other method. See: Jain S, Shukla S, Yang C, Zhang M, Fatma Z, Lingamaneni M, Abesteh S, Lande ST, Xiong X, Wang Y, Schroeder CM, Selvin PR, Zhao H (2021): TALEN outperforms Cas9 in editing herochromatin target sites. Nature communications, January 27.

Examples of crop irradiation from Japan including Fuji apples, the tandem accelerator in Fukui Prefecture; and Bayer Bioscience at Zwijnaarden.Reading time: 8 min

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